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Medical Laboratory Technician - MLT (ASCP) Practice Test

ASCP-MLT test Format | Course Contents | Course Outline | test Syllabus | test Objectives

Exam Specification:

- test Name: ASCP-MLT Medical Laboratory Technician - MLT (ASCP)
- test Code: ASCP-MLT
- test Duration: 3 hours and 30 minutes
- test Format: Multiple-choice questions
- Passing Score: Determined by the American Society for Clinical Pathology (ASCP)

Course Outline:

1. Hematology
- Blood cell morphology and identification
- Hemostasis and coagulation
- Hematological disorders and diseases
- Quality control and quality assurance

2. Clinical Chemistry
- Analytical techniques and instrumentation
- Enzymology and enzyme analysis
- Electrolyte and acid-base balance
- Endocrine and metabolic disorders

3. Microbiology
- Bacteriology and bacterial identification
- Mycology and fungal identification
- Parasitology and parasitic identification
- Virology and viral identification

4. Immunology and Serology
- Immune response and antibody-antigen reactions
- Serological testing methods
- Autoimmune diseases and immune disorders
- Transplantation immunology

5. Urinalysis and Body Fluids
- Urine analysis and microscopic examination
- Body fluid analysis (e.g.- cerebrospinal fluid- pleural fluid)
- Renal function and renal disorders
- Calculations and interpretations

6. Laboratory Operations
- Laboratory safety and biohazard handling
- Quality control and quality assurance
- Laboratory information systems
- Laboratory management and regulations

Exam Objectives:

1. Perform laboratory procedures accurately and efficiently.
2. Demonstrate knowledge of laboratory principles and practices.
3. Identify and troubleshoot laboratory instrument and test result issues.
4. Apply quality control measures to ensure accurate and reliable test results.
5. Recognize and interpret abnormal laboratory findings and their clinical significance.
6. Follow laboratory safety protocols and maintain a safe work environment.
7. Utilize laboratory information systems and effectively manage laboratory data.

Exam Syllabus:

The test syllabus covers the following Topics (but is not limited to):

- Hematology: blood cell morphology- coagulation- hematological disorders
- Clinical Chemistry: analytical techniques- electrolyte balance- metabolic disorders
- Microbiology: bacterial identification- fungal identification- viral identification
- Immunology and Serology: immune response- serological testing methods- autoimmune diseases
- Urinalysis and Body Fluids: urine analysis- body fluid analysis- renal function
- Laboratory Operations: safety protocols- quality control- laboratory management

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ASCP-MLT
Medical Laboratory Technician - MLT (ASCP)
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Question: 105
Class C fires involve:
Question options:
A. grease or oil
B. electrical equipment
C. wood
D. flammable materials
Answer: B
Question: 106
The intended response is Vitamin B12 and folate deficiencies. Each of these conditions lead to a megaloblastic
production of the red blood cells in the bone marrow. Since vitamin B12 and folate are needed in order to produce a
synchronous development of the nucleus with the cytoplasm in hematologic cells, oval-macrocytosis often occurs if
these nutrients are not in adequate supply within the body. This can also affect neutrophils, allowing for the
characteristic hypersegmented nucleus.
The photographic field contains several oval-macrocytes and a hypersegmented neutrophil with greater than 5 nuclear
segments. Oval macrocytes are most commonly associated with pernicious anemia and malabsorption syndromes
leading to vitamin B12 and folic acid deficiencies.
Clinical information relating to chronic infection, aplastic anemia, and other hematologic maligancies provide the
context for the presence of the oval macrocyte.
Macrocytic erythrocytes and hypersegmented neutrophils are not present in thalassemias or in Pelger-Huet anomaly
(hyposegmented neutrophils).
Conditions suggested by the macrocytes and the neutrophil in the photograph to the right
include which of the following?
A. Thalassemia
B. Vitamin B12 deficiency
C. Pelger-Huet anomaly
D. Folate deficiency
Answer: B,D
Question: 107
hs-CRP is a more sensitive version of the C-reactive protein (CRP) test, a test that has been used for many years to
assess inflammation in settings such as lupus, transplantation, infection, etc.
Which of the following cardiovascular risk markers is a more sensitive version of a test that is used to assess
inflammation?
A. Oxidized-LDL
B. hs-CRP
C. ApoB/ApoA1
D. LpPLA2
Answer: B
Question: 108
Which of the procedures listed below will increase the platelet concentration in the preparation of Platelets?
A. Centrifuge the blood at a low speed, remove the plasma and spin the plasma again at a low speed.
B. Centrifuge the bood at a low speed, remove the plasma and spin the plasma again at a
high speed.
C. Centrifuge the blood at a high speed, remove the plasma and spin the plasma again at a high speed.
D. Increase the duration of rotation
Answer: C
Question: 109
The production of long, tapered phialides is one of the key identifying features of Trichoderma species. In contrast,
Penicillium species, produce phialides with blunt ends.
The phialides of Beauveria species are geniculate, forming a zig-zag pattern.
The arrows in the image on the right point to single, long, tapered phialides that extend laterally from either side of the
hyphae. This is an identifying feature of which of the fungi listed below?
A. Trichoderma species
B. Penicillium species
C. Beauveria species
Answer: A
Question: 110
HbsAg is positive in acute and chronic Hepatitis B infections, since the antigen is found on the real surface of the
virus. HbeAg is present in the blood when the hepatitis B viruses are replicating, indicating an active infection. Anti-
Hbc IgM is present due to the immune response to the presence of the hepatitis core antigen and indicates an acute
infection. Anti-HBs is generally interpreted as indicating recovery and immunity from hepatitis B virus infection,
according to the CDC.
Given the following results, what is the immune status of the patient?
HbsAg: positive
HbeAg: positive
Anti-HBc IgM: positive
Anti-HBs: negative
A. acute infection
B. chronic infection
C. immunization
D. susceptible
Answer: A
Question: 111
Each of the drugs/drug classes listed above have been known to cause drug-induced hemolytic anemia, although
cephalosporins are the MOST COMMON cause. Cephalosporins can cause drug-induced hemolytic anemia when a
patient produces antibodies to the particular cephalosporin drug in the presence of red blood cells. The drugs can alter
the membrane appearance of the red blood cells, causing the body to mistake them as foreign. Complement becomes
activated due to these antibodies; red cells are then destroyed causing hemolytic anemia. Dark urine, caused by
intravascular hemolysis, is one of the most common symptoms associated with this condition.
Which one of the following drugs/drug classes is the MOST COMMON cause of drug-induced hemolytic anemia?
A. Levodopa
B. Quinidine
C. Cephalosporins
D. Levofloxacin
E. Nonsteroidal anti-inflammatory drugs (NSAIDs)
Answer: C
Question: 112
The tube of choice for trace metal analysis is:
A. red
B. royal blue
C. gold
D. light blue
Answer: B
Question: 113
Acute hemolytic transfusion reactions are most commonly due to ABO-incompatible blood being transfused to a
recipient with naturally occurring ABO alloantibodies (anti-A, anti-B, anti A,B).
Acute intravascular hemolysis as the result of a blood transfusion is most often associated with which of the following
causes?
A. Transfusion of ABO incompatible red cells
B. Allergies
C. Passively transfused antibodies to HLA antigens
D. Transfusion-associated graft-versus-host disease
Answer: A
Question: 114
Lower right image
Chains of large, lemon-shaped annelloconidia
A. Aspergillus niger
B. Microsporum canis
C. Scopulariopsis species
D. Acremonium species
Answer: A,B,C,D
Question: 115
Only non-self antigens can be immunogenic. Self antigens are normally recognized by the immune system as part of
the host, so an immune response does not normally occur. Non-self antigens are immunogenic since they have the
potential to cause an immune response.
For a substance to be immunogenic it must be:
A. A linear molecule
B. A lipid
C. Less than 5,000 molecular weight
D. Recognized as non-self
E. A haptene
Answer: D
Question: 116
Gating sets up boundaries around a subset of data points to segregate those events for analysis.
Gating the proper cell population is crucial for flow cytometric analysis. The CD marker information presented on the
histogram data is only representative of the cells inside the gate.
When analyzing the raw data of a sample on the flow cytometer, CD marker information on the histograms represents
data inside the _____ population.
A. Gated
B. Non-gated
Answer: A
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